The regions of the herpes simplex virus type 1 immediate early protein Vmwl75 required for site specific DNA binding closely correspond to those involved in transcriptional regulation

The immediate-early (IE) protein Vmw175 (ICP4) of HSV-1 is required for the transcription of later classes of viral genes and the repression of IE gene expression. We have previously constructed a panel of plasmid-borne insertion and deletion mutants of the gene encoding Vmw175 and assayed their ability to regulate transcription in transient transfection assays. By this approach we have mapped the regions of the Vmw175 amino acid sequence that are required for transcriptional activation and repression of herpes virus promoters. This paper describes the use of nuclear extracts, made from cells transfected with these mutant plasmids, in gel retardation DNA binding assays in order to define the regions of Vmw175 involved in binding to a specific Vmw175 DNA binding site. The results show that amino acid residues 275–495 (a region which is highly conserved between Vmw175 and the varicella-zoster virus “IE” 140K protein) include structures which are critically required for specific DNA binding, transactivation and repression. This raises the interesting paradox that although the specific DNA sequence recognized by Vmw175 is not commonly found in its target promoters, the protein domain required for recognition of this sequence is required for promoter activation

The major transcriptional regulatory protein of herpes simplex virus type 1 includes a protease resistant DNA binding domain

Herpes simplex virus type 1 expresses five immediateearly (IE) polypeptides. In the absence of functional Vmw175 (the product of IE gene 3) activation of transcription of later classes of viral genes and repression of IE gene expression does not occur. The recognition of specific DNA sequences by Vmw175 requires, as determined by sensitivity to mutation, a part of the protein highly conserved in the corresponding proteins of related herpes viruses. However, mutations in other parts of the protein can also disrupt specific DNA binding. This paper shows that the DNA binding domain of Vmw175 can be liberated as a functional unit by digestion with proteinase K. Analysis of mutant Vmw175 proteinsshowed that the proteinase K resistant domain has an amino terminus between amino acid residues 229 and 292, while its carboxy terminus is between residues 495 and 518. Mutations outside this region which affect DNA binding by the intact protein do not eliminate binding of the proteinase K resistant domain. This implies that direct DNA binding by Vmw175 involves a linear subsection of the polypeptide, and that mutations in other parts of the polypeptide which affect DNA binding of the whole protein do so by indirect means

Satellite lifetime routine user's manual

A FORTRAN coded computer program which determines secular variations in mean orbital elements of earth satellites and the lifetime of the orbit is described. The dynamical model treats a point mass satellite subject to solar and lunar disturbing gravitational fields, second, third and fourth harmonics of the earth's oblate potential, earth's atmospheric drag, and solar radiation pressure. Each of these disturbing functions may be selectively simulated. Data preparation instructions, a sample problem, and definitions of output quantities are included

Routine Immunizations in Chester, VT

https://scholarworks.uvm.edu/fmclerk/1393/thumbnail.jp

Breeding Biology of the Black Storm-Petrel at Islas Los Coronados, Baja California, Mexico

Twenty-nine visits to Middle Rock, Islas Los Coranados, were made between March 1989 and September 1990, including 10 overnight visits. Breeding phenology was studied, including timing of colony attendance by adult birds, timing of egg laying, hatching, and fledging. Over 200 birds were banded to determine nest site tenacity and pair bond retention. Details were recorded on nest site selection, interspecific competition, breeding success, chick growth and plumage development, adult morphometrics, molt, food items, vocalizations, and ectoparasites

Dirac Neutrino Masses from Generalized Supersymmetry Breaking

We demonstrate that Dirac neutrino masses in the experimentally preferred range are generated within supersymmetric gauge extensions of the Standard Model with a generalized supersymmetry breaking sector. If the usual superpotential Yukawa couplings are forbidden by the additional gauge symmetry (such as a U(1)'), effective Dirac mass terms involving the "wrong Higgs" field can arise either at tree level due to hard supersymmetry breaking fermion Yukawa couplings, or at one-loop due to nonanalytic or "nonholomorphic" soft supersymmetry breaking trilinear scalar couplings. As both of these operators are naturally suppressed in generic models of supersymmetry breaking, the resulting neutrino masses are naturally in the sub-eV range. The neutrino magnetic and electric dipole moments resulting from the radiative mechanism also vanish at one-loop order.Comment: 5 pages, 1 figure, references added, note added on effective superpotential mass terms generated upon U(1)' breakin

Tecumseh\u27s Young Braves A Story of the Creek War

https://commons.und.edu/settler-literature/1046/thumbnail.jp